1.
W. Kelley Thomas, Ph.D.
WILLIAM KELLEY THOMAS Hubbard Professor in Genomics and Director ... Durham, NH 03824. Phone: 603-862-2470 Fax: 603-862-2940. Email: kelley.thomas@unh.edu ...
2.
Size standards and dyes
It is optimal to load 0.5 ug of size standard in a gel. If you make up the size standard in loading dye to the following recipe, load 10 ul to get 0.5 ug. ...
3.
Agar plates
Pouring plates. 1. Prepare the media. Prepare 1 liter of LB agar. Use a 2 liter flask and add 1 l of distilled water from the DW tap at the sink. ...
4.
CA primer design
It may be necessary to sequence the clone with the reverse primer as clones ... >100 bp for designing a reverse primer) copy the sequence into the database. ...
5.
Weimin Ye-Publications
Anguina agrostis (Steinbuch, 1799) Filipjev, 1936 found in Shenzhen, China associated with imported seeds of weeping love grass. ...
6.
Acryalmide gels on ABI 377
Amberlite MB-150 resin, with other chemicals under A ... Add 180 g of Urea, 5 g of Amberlite MB-150 Ion Exchange Resin, and 50 ml of 40% acrylamide/bis ...
7.
000
File Format: PDF/Adobe Acrobat - View as HTML becril: a mullispecics con- glomerate? Copcia 1980:938-941. [■"l•lil-R, M. P.. 1977. Oxygen consumption and activity in salamanders: effect of bode si/c ...
8.
Degenerate primer design
One last trick is to add tails to the degenerate primers on the 5' ends. This helps to increase the PCR efficiencies of these primers by increasing primer ...
9.
Degenerate primer design
The next task is to determine which of the possible primers are the least degenerate. Looking at the amino acid code, some amino acids are coded for by more ...
10.
Real time PCR
Real time PCR and real time RT-PCR are a powerful way to quantify relative amounts of ... The basic steps to a real time experiment to look at relative gene ...
