1.
FICOLL ISOPAQUE TECHNIQUE FicoU-Isopaque Reagent 1. 6.48gm Ficoll ...
File Format: PDF/Adobe Acrobat - View as HTML FicoU-Isopaque Reagent. 1. 6.48gm Ficoll + 10.17 gm Isopaque dissolved in 90 ml H20, then q.s. to 102 ml. Lasts at least one month, d = 1.077gm/cc ...
2.
ELISA SUBSTRATE BUFFER (ESB)
ELISA SUBSTRATE BUFFER (E.S.B.). Contact: Ometa Herman. 0.05M Sodium Phosphate. 0.02M Citric Acid. MAKE UP. 0.2M Sodium Phosphate. Na2HPO4 FW142.00 ...
3.
PBMC Prep Protocol
PBMC Preparation from Buffy Coats. Last Updated 11/30/00. Contacts: Steve De Rosa (derosa@fhcrc.org) Ometa Herman (ometa@stanford.edu ) ...
4.
Antibody titration analsis
Creation of a table consisting of median positive and negative fluorescences versus antibody titre which can then be exported into Kaleidegraph or other ...
5.
Photogenerated glycan arrays identify immunogenic sugar moieties ...
File Format: PDF/Adobe Acrobat - View as HTML Photogenerated glycan arrays recognize immunogenic sugar moieties of the B. anthracis spore. A panel of 35 mono-, oligo-, and ...
6.
John Mantovani - Herzenberg Laboratory
John is responsible for the set-up and maintenance of this IIS as well as ... De Rosa, S. C., J. P. Andrus, S. P. Perfetto, J. J. Mantovani, ...
7.
Intracellular Cytokine FACS-Staining Protocol
Nov 17, 1999 ... Intracellular staining. Resuspend in 25 ml per well of permeabilization buffer containing intracellular Abs(cytokine, perforin) ...
8.
Antibody titration analsis
This protocol is to be used specifically in conjunction with FlowJoTMflow cytometric data analysis software and Kaleidegraph data anlaysis and graphing ...
9.
Intracellular Cytokine FACS-Staining Protocol
Nov 17, 1999 ... For HIV samples, this final resuspension should be in staining media (without serum) containing 0.5% paraformaldehyde. FACS analysis ...
10.
Chapter 109 Hybridoma immunoglobulin isotype switch variant ...
File Format: PDF/Adobe Acrobat - View as HTML The ability to select switch variant hybridomas producing monoclonal antibody with a particular immunoglobulin isotype may greatly enhance the application ...
