1.
duckfeet: CRP and Sxy plans
I now feel that the manuscript should be only about CRP and that experiments testing Sxy’s role in DNA binding and transcription activation should be saved ...
2.
duckfeet: Testing whether the CRP-S sequence confers Sxy-dependence.
They depend on the Sxy protein for transcription, and 2. The CRP-binding sites (called “CRP-S”) in these promoters differ at the sequence level from other ...
3.
duckfeet: Testing whether the CRP-S sequence confers Sxy-dependence.
They depend on the Sxy protein for transcription, and 2. The CRP-binding sites (called “CRP-S”) in these promoters differ at the sequence level from other ...
4.
duckfeet: Latest ICAP results
Latest ICAP results. I have now quantified EcCRP and HiCRP affinity for ICAP and four designer variants, and am currently replicating the experiments. ...
5.
duckfeet: RNA becometh DNA day
RNA becometh DNA day. Today I destroy some DNA and make some more (ie. DNase treat my RNA samples and then reverse transcribe them). ...
6.
duckfeet: Hot stuff (aka. sticky Sxy)
Nov 8, 2007 ... Hot stuff (aka. sticky Sxy). After a grueling few days of bandshift experiments to study CRP and Sxy binding to the pilA promoter, ...
7.
duckfeet: Trying to make T7 RNAP behave like bacterial RNAP
A central complication is that the the in vitro assay uses the phage T7 RNA polymerase to achieve high levels of transcription. T7 RNAP is a very fast and ...
8.
duckfeet: Trying to make T7 RNAP behave like bacterial RNAP
A central complication is that the the in vitro assay uses the phage T7 RNA polymerase to achieve high levels of transcription. T7 RNAP is a very fast and ...
9.
duckfeet: The complexities of gene regulation in E. coli
The complexities of gene regulation in E. coli ... whereas bacteria in more complex environments employ a much larger number of regulators. ...
10.
duckfeet: Testing whether the CRP-S sequence confers Sxy-dependence.
Next I mutated the CRP site in the lacZ promoter; this was easy because our standard ... I then cloned the lacZ promoter mutant in E. coli, but because Sxy ...
