1.
Knockouts and RNAi.ppt
File Format: PDF/Adobe Acrobat - View as HTML (contain both transplanted ES cells and host cells). • Mice each heterozygous for the knockout mutation are mated to produce homozygous knockout mice ...
2.
Knockouts and RNAi.ppt
File Format: PDF/Adobe Acrobat - View as HTML RNAi first used in C. elegans as a response to double-stranded RNA which resulted in sequence- ... “RNAi functions in cultured mammalian neurons” ...
3.
Protocol for siRNA annealing and storage
File Format: PDF/Adobe Acrobat - View as HTML Annealing of siRNA. Scientists are sometimes puzzled when the time to choose the right protocol has come. Based on this observation, Eurogentec’s RNA ...
4.
Microsoft PowerPoint - siRNA.ppt
File Format: PDF/Adobe Acrobat - View as HTML Inducing RNAi in Mammalian Cells. View at: www.ambion.com/techlib/presentations. siRNA localized to the perinuclear space. Important Applications of RNAi ...
5.
Protocol for siRNA annealing and storage
File Format: PDF/Adobe Acrobat - View as HTML up to 5 times. A siRNA duplex is formed by hybridizing sense and antisense complementary RNA oligonucleotides. Protocol for siRNA annealing and storage ...
6.
Knockouts and RNAi.ppt
File Format: PDF/Adobe Acrobat - View as HTML RNAi first used in C. elegans as a response to double-stranded RNA which resulted in sequence- ... “RNAi functions in cultured mammalian neurons” ...
7.
Microsoft PowerPoint - siRNA.ppt
File Format: PDF/Adobe Acrobat - View as HTML Cocktail of several siRNAs generated by RNase III/Dicer digestion of long dsRNA ... Cocktail of siRNAs provides better chance for strong RNAi effect on ...
8.
Established Transfection Reagent for Mammalian Cells 1. General ...
DOTAP is a monocationic transfection reagent based on liposome technology. ... The solutions of DNA and DOTAP transfection reagent should have ambient ...
9.
Protocol for siRNA annealing and storage
File Format: PDF/Adobe Acrobat - View as HTML Annealing of siRNA. Scientists are sometimes puzzled when the time to choose the right protocol has come. Based on this observation, Eurogentec’s RNA ...
10.
Rational siRNA design for RNA interference
File Format: PDF/Adobe Acrobat the predicted melting temperature of the siRNA hair- pin loop, was calculated based on nearest neighbor method. 11 using Oligo 6.0 software package. ...
